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Two non-invasive chemical imaging modalities were employed to help understand the changes made over time to the Lamb of God, the focal point of the Ghent Altarpiece (1432) by Hubert and Jan Van Eyck. Two major results were obtained: a prediction of the facial features of the Lamb of God that had been hidden beneath nonoriginal overpaint dating from the 16th century (and later), and evidence for a smaller earlier version of the Lamb’s body with a more naturalistic build.